Maternal Cell Contamination
|Repeat Expansion/Fragment Length Analysis
|2-4 ml of blood with anticoagulant EDTA from mother
30-50 ng DNA in TE, AE or pure sterile water at 100-250 ng/µl from mother
30-50 ng DNA in TE, AE or pure sterile water at 100-250 ng/µl from fetusThe A260/A280 ratio should be 1.8-2.0. DNA sample should be run on an agarose gel as a single band, showing no degradation, alongside with a quantitative DNA marker.
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