Hypolactasia – Lactose intolerance hereditary predisposition analysis

Genes
(full coding
region):		 LCT gene polymorphism 13910C>T analysis
Lab method: Sanger sequencing
TAT: 1-2 weeks
Specimen requirements: Sterile buccal swab.

2-4 ml of blood with anticoagulant EDTA.

1 µg DNA in TE, AE or pure sterile water at 100-250 ng/µl
The A260/A280 ratio should be 1.8-2.0. DNA sample should be run on an agarose gel as a single band, showing no degradation, alongside with a quantitative DNA marker.
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