{"id":29138,"date":"2024-03-24T18:02:07","date_gmt":"2024-03-24T16:02:07","guid":{"rendered":"https:\/\/www.asperbio.com\/?page_id=29138"},"modified":"2024-03-24T18:02:47","modified_gmt":"2024-03-24T16:02:47","slug":"munasarjavahiga-seotud-geenide-sekveneerimine","status":"publish","type":"page","link":"https:\/\/www.asperbio.com\/et\/asper-oncogenetics-testid\/munasarjavahiga-seotud-geenide-sekveneerimine\/","title":{"rendered":"Munasarjav\u00e4higa seotud geenide sekveneerimine"},"content":{"rendered":"

Munasarjav\u00e4higa seotud geenide sekveneerimine<\/span><\/h2>\n
\n\n\n\n
Geenid:<\/strong><\/td>\nBRCA1, BRCA2, BRIP1, PALB2, RAD51C, RAD51D<\/em>
\n<\/em><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n\n\n\n
Metoodika:<\/strong><\/td>\nKodeeriva piirkonna sekveneerimine (NGS).
\nKoopiaarvu muutuste bioinformaatiline anal\u00fc\u00fcs (CNV). CNV leidude kinnitamine teise meetodiga toimub lisaanal\u00fc\u00fcsina, vastavalt hinnakirjale.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n\n\n\n
Testi valmimisaeg:<\/strong><\/td>\n6-9\u00a0n\u00e4dalat<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n\n\n\n
N\u00f5uded proovi-materjalile:<\/strong><\/td>\n2-4 ml t\u00e4isverd antikoagulandiga EDTA (lilla korgiga katsuti)<\/p>\n

1\u00a0\u00b5g DNA-d elueerituna TE, AE puhvris v\u00f5i steriilses vees, kontsentratsiooniga 100-250 ng\/\u00b5l
\nDNA saata toatemperatuuril v\u00f5i k\u00fclmutatuna. A260\/A280 suhe peaks olema 1.8-2.0. DNA peab agaroosgeelis pikkusmarkeri juuresolekul olema detekteeritav \u00fche tervikliku b\u00e4ndina.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\n\n\n\n
Tellimine:<\/strong><\/td>\nProovimaterjal saata koos saatekirjaga<\/strong><\/span><\/a> Asper Biogene laborisse<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n<\/div>\n
\n

N\u00e4idustused geenitesti tegemiseks:<\/strong><\/p>\n

    \n
  1. Rinna- v\u00f5i munasarjav\u00e4hk noores eas: patsient on haiguse diagnoosimisel noorem kui 50 aastane<\/li>\n
  2. Haigusseoselise mutatsiooni leiu puhul on vajalik l\u00e4hisugulaste testimine<\/li>\n
  3. Suguv\u00f5sas on esinenud rinna- v\u00f5i munasarjav\u00e4hki<\/li>\n
  4. Suguv\u00f5sas on esinenud meeste rinnav\u00e4hki<\/li>\n
  5. Geneetiline n\u00f5ustamine<\/li>\n<\/ol>\n

    Munasarjav\u00e4hki<\/strong> seostatakse enim mutatsioonidega BRCA1<\/em> ja BRCA2<\/em> geenides. Erinevatel mutatsioonidel on v\u00e4hi tekkes erinev roll: m\u00f5ni neist on \u00fcsna n\u00f5rgalt seotud v\u00e4hi tekkega, teised mutatsioonid lubavad aga ennustada kuni 80% v\u00e4hiriski t\u00f5usu. Mutatsioonide esinemissagedus on populatsiooniti m\u00f5nev\u00f5rra erinev.<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

    Munasarjav\u00e4higa seotud geenide sekveneerimine Geenid: BRCA1, BRCA2, BRIP1, PALB2, RAD51C, RAD51D Metoodika: Kodeeriva piirkonna sekveneerimine (NGS). Koopiaarvu muutuste bioinformaatiline anal\u00fc\u00fcs (CNV). CNV leidude kinnitamine teise meetodiga toimub lisaanal\u00fc\u00fcsina, vastavalt hinnakirjale. Testi valmimisaeg: 6-9\u00a0n\u00e4dalat N\u00f5uded proovi-materjalile: 2-4 ml t\u00e4isverd antikoagulandiga EDTA (lilla korgiga katsuti) 1\u00a0\u00b5g DNA-d elueerituna TE, AE puhvris v\u00f5i steriilses vees, kontsentratsiooniga 100-250 ng\/\u00b5l […]<\/p>\n","protected":false},"author":11,"featured_media":0,"parent":16918,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-29138","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.asperbio.com\/et\/wp-json\/wp\/v2\/pages\/29138","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.asperbio.com\/et\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.asperbio.com\/et\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.asperbio.com\/et\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/www.asperbio.com\/et\/wp-json\/wp\/v2\/comments?post=29138"}],"version-history":[{"count":2,"href":"https:\/\/www.asperbio.com\/et\/wp-json\/wp\/v2\/pages\/29138\/revisions"}],"predecessor-version":[{"id":29141,"href":"https:\/\/www.asperbio.com\/et\/wp-json\/wp\/v2\/pages\/29138\/revisions\/29141"}],"up":[{"embeddable":true,"href":"https:\/\/www.asperbio.com\/et\/wp-json\/wp\/v2\/pages\/16918"}],"wp:attachment":[{"href":"https:\/\/www.asperbio.com\/et\/wp-json\/wp\/v2\/media?parent=29138"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}