{"id":12026,"date":"2015-11-10T11:26:39","date_gmt":"2015-11-10T11:26:39","guid":{"rendered":"http:\/\/www.asperbio.com\/?page_id=12026"},"modified":"2018-03-19T14:48:05","modified_gmt":"2018-03-19T12:48:05","slug":"perekondlik-adenomatoosne-polupoos","status":"publish","type":"page","link":"https:\/\/www.asperbio.com\/et\/asper-oncogenetics-testid\/perekondlik-adenomatoosne-polupoos\/","title":{"rendered":"Perekondlik adenomatoosne pol\u00fcpoos"},"content":{"rendered":"

Adenomatoosse pol\u00fcpoosiga seotud APC<\/em> geeni sekveneerimine<\/span><\/h2>\n
\n\n\n\n
Geenid:<\/strong><\/td>\nAPC<\/em><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n\n\n\n
Metoodika:<\/strong><\/td>\nKodeeriva piirkonna sekveneerimine (Sanger)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n\n\n\n
Testi valmimisaeg:<\/strong><\/td>\n2-4 n\u00e4dalat<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n\n\n\n
N\u00f5uded proovi-materjalile:<\/strong><\/td>\n2-4 ml t\u00e4isverd antikoagulandiga EDTA (lilla korgiga katsuti)<\/p>\n

2 \u00b5g DNA-d elueerituna TE, AE puhvris v\u00f5i steriilses vees, kontsentratsiooniga 100-250 ng\/\u00b5l
\nDNA saata toatemperatuuril v\u00f5i k\u00fclmutatuna. A260\/A280 suhe peaks olema 1.8-2.0. DNA peab agaroosgeelis pikkusmarkeri juuresolekul olema detekteeritav \u00fche tervikliku b\u00e4ndina.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\n\n\n\n
Tellimine:<\/strong><\/td>\nProovimaterjal saata koos saatekirjaga<\/strong><\/span><\/a> Asper Biogene laborisse<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n<\/div>\n

APC<\/em> geeni deletsioonide\/duplikatsioonide anal\u00fc\u00fcs<\/span><\/h2>\n
\n\n\n\n
Geenid:<\/strong><\/td>\nAPC<\/em><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n\n\n\n
Metoodika:<\/strong><\/td>\nMLPA<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n\n\n\n
Testi valmimisaeg:<\/strong><\/td>\n4-6 n\u00e4dalat<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n\n\n\n
N\u00f5uded proovi-materjalile:<\/strong><\/td>\n2-4 ml t\u00e4isverd antikoagulandiga EDTA (lilla korgiga katsuti)<\/p>\n

1 \u00b5g DNA-d elueerituna TE, AE puhvris v\u00f5i steriilses vees, kontsentratsiooniga 100-250 ng\/\u00b5l
\nDNA saata toatemperatuuril v\u00f5i k\u00fclmutatuna. A260\/A280 suhe peaks olema 1.8-2.0. DNA peab agaroosgeelis pikkusmarkeri juuresolekul olema detekteeritav \u00fche tervikliku b\u00e4ndina.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\n\n\n\n
Tellimine:<\/strong><\/td>\nProovimaterjal saata koos saatekirjaga<\/strong><\/span><\/a> Asper Biogene laborisse<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
\n<\/div>\n

N\u00e4idustused geenitesti tegemiseks:<\/strong><\/p>\n

    \n
  1. Patsientidele, kellel on tuvastatud adenomatoossed pol\u00fc\u00fcbid<\/li>\n
  2. Adenomatoossete pol\u00fc\u00fcpidega patsientide l\u00e4hisugulaste testimiseks<\/li>\n
  3. Geneetiline n\u00f5ustamine<\/li>\n<\/ol>\n

    Perekondlik adenomatoosne pol\u00fcpoos<\/strong> (familial adenomatous polyposis<\/em>, FAP) esineb umbes 1% kolorektaalv\u00e4hi patsientidest. P\u00e4riliku adenomatoosse pol\u00fcpoosi puhul v\u00f5ib k\u00e4\u00e4r- ja p\u00e4rasooles moodustuda sadu pol\u00fc\u00fcpe. FAP s\u00fcndroomi iseloomustab autosoom-dominantne p\u00e4randumine ning see on p\u00f5hjustatud muutustest APC<\/em> geenis.<\/p>\n

    Ligikaudu 8% APC<\/em> geeni mutatsioonidega patsientidel kujuneb v\u00e4lja p\u00e4riliku adenomatoosse pol\u00fcpoosi nn leebem vorm, attenuated<\/em> FAP (AFAP). AFAP-patsiente iseloomustab j\u00e4mesooles v\u00e4iksem pol\u00fc\u00fcpide arv (<100). Samuti kujuneb kolorektaalv\u00e4hk selle s\u00fcndroomi korral v\u00e4lja m\u00e4rksa hilisemas eas.<\/p>","protected":false},"excerpt":{"rendered":"

    Adenomatoosse pol\u00fcpoosiga seotud APC geeni sekveneerimine Geenid: APC Metoodika: Kodeeriva piirkonna sekveneerimine (Sanger) Testi valmimisaeg: 2-4 n\u00e4dalat N\u00f5uded proovi-materjalile: 2-4 ml t\u00e4isverd antikoagulandiga EDTA (lilla korgiga katsuti) 2 \u00b5g DNA-d elueerituna TE, AE puhvris v\u00f5i steriilses vees, kontsentratsiooniga 100-250 ng\/\u00b5l DNA saata toatemperatuuril v\u00f5i k\u00fclmutatuna. A260\/A280 suhe peaks olema 1.8-2.0. DNA peab agaroosgeelis pikkusmarkeri juuresolekul […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":16918,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-12026","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.asperbio.com\/et\/wp-json\/wp\/v2\/pages\/12026","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.asperbio.com\/et\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.asperbio.com\/et\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.asperbio.com\/et\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.asperbio.com\/et\/wp-json\/wp\/v2\/comments?post=12026"}],"version-history":[{"count":3,"href":"https:\/\/www.asperbio.com\/et\/wp-json\/wp\/v2\/pages\/12026\/revisions"}],"predecessor-version":[{"id":21682,"href":"https:\/\/www.asperbio.com\/et\/wp-json\/wp\/v2\/pages\/12026\/revisions\/21682"}],"up":[{"embeddable":true,"href":"https:\/\/www.asperbio.com\/et\/wp-json\/wp\/v2\/pages\/16918"}],"wp:attachment":[{"href":"https:\/\/www.asperbio.com\/et\/wp-json\/wp\/v2\/media?parent=12026"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}