GENORAMA^® GENOTYPING PLATFORM

The Genorama^® Genotyping Platform is a flexible, robust and affordable mutation detection platform suitable for arrayed primer extension (APEX) based mutation detection. All components of the Asper’s proprietary platform have been specifically designed and tested for APEX based applications. Please click here to read more about the platform…

Asper offers two different packages of the platform:
 

I STANDARD GENORAMA^® PACKAGE
 
Standard Genorama^® package includes the four colour instrument, the relevant software, set-up of the instrument and basic training.

II APEX PACKAGE

Asper has specifically established a package for APEX users, consisting of additional specific gear and instrumentation required for successfully performing APEX based testing. Asper strongly recommends to purchase the APEX package along with the platform, however it can also be shipped later.

ADDITIONAL PRODUCTS AND SERVICES RELATED TO GENORAMA^® GENOTYPING PLATFORM

 
Ready-to-use chips for Genorama^® Genotyping Platform

Asper has developed in collaboration with it’s scientific partners a number of tests targeting various conditions. The Genorama® platform owners can take advantage of those tests and run those on their instruments. The entire list of the existing tests (chips) can be found here. 

   Thalassemia testing
   Hereditary Hearing Loss testing
   Cystic Fibrosis testing
   DNA repair testing
   Ashkenazi Jewish (AJ) diseases testing
   Wilson disease testing
   Hereditary breast and ovarian cancer testing
   ABCR genetic testing
   Usher syndrome testing 
   Leber congenital amaurosis (LCA) genetic testing    
   Autosomal recessive retinitis pigmentosa(AR-RP) genetic testing
   Autosomal dominant retinitis pigmentosa (AD-RP) genetic testing  
   Bardet Biedl syndrome (BBS) genetic testing
   Autosomal dominant optic atrophy (ADOA) genetic testing
   Corneal dystrophy genetic testing
   Congenital stationary night blindness genetic testing
 
  

Please click on the test’s name to read more about it…
The summary table of entire list of the existing tests (chips) with the number of genes and positions can be found here. 

The list itself is expanding two or three times a year so please visit the page again in a few months.

Re-design of ready-to-use chips

In case the existing tests does not contain all the mutations of interest, Asper Biotech is ready to customize the tests by including additional mutations. 

Custom test development

Beside the customization of existing tests, the totally new test development is also provided. Leave the design, development and validation for specialists - Asper’s project managers use their more than seven years of experience in development of various APEX based tests and establish test for you, completely tailor made.
Asper’s spotting facility would order the oligos, store them and spot high-quality slides for you during and after the development process. There are no large minimum requirements, so you have in between spotting room for redesign and you’ll get after the next spotting run completely new version of your test. 

If specific tests are required, APEX is a platform to choose because of it’s robustness – it is easy and affordable to establish custom tests for testing tens of genes in one reaction.

Asper needs the following information and materials to start the custom chip development

SNP analysis
- SNP ID numbers (rs#, http://snepper.chip.org)
- DNA concentration and method of extraction
- If the SNP is not available in public databases we need sequence of 
              - 400 bp flanking area (if you send us genomic DNA)
              - 25 bp flanking area (if you send us a PCR product). 
NB! PCR must be performed according to our protocol in order to ensure incorporation of a small fraction of dUTP into your PCR products for fragmentation.)
- location/sequence of the PCR primers in case you already have designed them
- in case you order chip validation, please send us appropriate DNAs, one per each SNP on the chip and including information about the SNP in the sample.
 
Mutation analysis
- location of the mutation with 
           - 400 bp flanking sequence, if you send us genomic DNA
           - 25 bp flanking sequence, if you send us a PCR product. 
NB! PCR must be performed according to our protocol in order to ensure incorporation of a small fraction of dUTP into your PCR products for fragmentation.
- DNA concentration and method of extraction
- location/sequence of the PCR primers in case you already have designed them
- in case you order chip validation, please send us appropriate DNAs, one per each mutation on the chip and including information about the mutation in the sample

Genorama^® Microarray slides

Genorama^® Microarray Slides are developed and tested primarily for DNA-based applications. However, nucleic acids, proteins, small molecules, cells and extracts can also be immobilized. The activated glass slides are produced under rigorous quality control and tested for low background and good immobilization characteristics. As a result, the slides have a very clean and uniform surface, highly suitable for printing top quality microarrays. The microarray slides have a double-sided coating with a printable area of 23 x 73 mm.

Please click here to read more about the slides…

Custom spotting service

Asper Biotech offers a complete solution for custom microarray spotting, including array design for APEX genotyping or providing oligos through its partners. The know-how and experience at Asper helps researchers achieve the best quality microarrays. We use VersArray ChipWriter Pro (BioRad) and GMS Arrayer 417 (Affymetrix).

Main features of the service
- High quality (uniform) spot
- Spot diameter 90-100 micrometers
- Minimal distance between spots 150 micrometers (optimal 250-375 micrometers)
- Asper uses Telechem SMP3 pins
- Printing in controlled environment
- Asper Biotech recommends high-quality and high-performance Genorama^® microarray slides.
 

Download the brochure about Genorama^® Genotyping platform (770 kb)

Please find more information on individual parts of the platform below: 

Genorama^® QuattroImager
Genorama^® Genotyping Software
SNP Assistant 
Genorama^® Microarray slides and spotting solutions