4 colour microarray sanner for genotyping

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GENORAMA^® QUATTROIMAGER^TM

Genorama^® QuattroImager^TM is a four-channel microarray imaging and analysis system, specifically designed for repetitive analysis of multiple slides. International patent application is filed for Genorama^® QuattroImager^TM microarray imaging system.

The working principle of the system is based on total internal reflection fluorescence excitation mechanism combined with a charge coupled device (CCD) detector. The signals from the spectrally separated dyes are resolved with dual selection by laser excitation and band-pass filtering of the emitted fluorescence.

At low penetration angles, total internal reflection results when light propagating within a dense medium (e.g. glass) reaches an interface with a less dense medium (e.g. air or aqueous solution). Although the light is fully reflected, an evanescent field is generated that extends beyond the interface and into the less dense medium.

Typically, the penetration depth (or the thickness of evanescent field) is in the range of half the wavelength of the light. Only fluorophores bound to the surface will be excited and therefore fluoresce contributing to an enhanced signal to noise ratio. The fluorescence signals from the spectrally separated dyes are resolved with the dual selection by monochrome laser excitation and narrow band-pass filtering of the emitted fluorescence.

Imaging is followed by software analysis to convert the acquired fluorescence image into the sequence data.

COMPONENTS OF GENORAMA

1. Cooled Digital CCD camera System with 2184 x 1472 pixels (pixel size 6.8 x 6.8 µm).
2. Six position motorized filter wheel
3. Camera lens
4. Optical tube
5. Slide chamber and laser beam inlet
6. Lens for focusing the laser beam
7. Common light path with shutters and reflector mirrors

Genorama QuattroImager will be delivered with a preinstalled Windows XP computer. Customer can choose between desktop and notebook.

SPECIFICATIONS

Array substrate	24 x 60 mm coverslips
Imaging field	22 x 32 mm
Resolution	15 µm
Sensitivity	5-10 molecules fluor/mm²
Dynamic range	16 bit
Excitation Wavelengths	4 lasers 635 nm Diode laser 594 nm DPSS laser 532 nm DPSS laser 473 nm DPSS laser
Emission Wavelengths	6 position filter wheel with narrow band pass filters 670 nm 620 nm 570 nm 520 nm (other wavelengths on request)
Sensor	CCD camera
Pixel array	2184x1472
Noise reduction	Active, Peltier cooling
Sensor operating temperature	-25° C
Computer	Standard Pentium PC
Size (W x D x H)	55 x 45 x 22 cm
Weight	30 kg
Electrical	100 ... 240 VAC, 50 ... 60 Hz

THE ADVANTAGES

	the short imaging time (the image acquisition per channel (excitation wavelength) takes from 10 to 60 seconds)
	different custom built configurations available
	The modular system can be easily altered to need the excitation /detection of a wide selection of fluorescent markers
	unlike confocal microscopy there is no need for optically flat polished slides
	Unique Genorama^{^®}GenotypingSoftware, which enables to analyze microarray based sequence data is accompanied with detector
	Full service: Asper will set up the genotyping method APEX in customer's lab.
	Affordable price
	Many ready-to-use applications

For more information, including pricing, please contact info@asperbio.com.

GENETIC TESTS

   Thalassemia testing

   Hereditary Hearing Loss
    testing

   Cystic Fibrosis testing

   DNA repair testing

   Ashkenazi Jewish (AJ)
    diseases testing

   Wilson Disease testing

   Hereditary Breast and
    Ovarian Cancer testing

   Stargardt disease, age
    related macular
    dystrophy, cone-rod
    dystrophy testing

   Usher syndrome testing

   Leber congenital
    amaurosis (LCA) genetic
    testing

   Autosomal recessive
    retinitis pigmentosa
    (AR-RP) genetic testing

   Autosomal dominant
    retinitis pigmentosa
    (AD-RP) genetic testing

   Bardet Biedl syndrome
    (BBS) genetic testing

   Autosomal dominant
    optic atrophy testing

   Corneal dystrophy
    testing

   Congenital stationary
    night blindness
    genetic testing

Vitelliform macular
    dystrophy testing

MICROARRAY SLIDES

   Genorama^® SAL

   Genorama^® SA

Spotting order FORM

Sample submission FORM

ISO 9001 quality standard