Requirements for samples
Asper Biotech accepts extracted DNA and blood for genetic examination. For the tests like AMD risk assessment, Apo E genotyping, Lactose Intolerance, resistance to norovirus infections, sufficient DNA can be processed from saliva. Preferred saliva collection kits are Oragene DNA and Invitek Saligene.
Requirements for DNA samples
>> Genomic DNA should be eluted in pure sterile water, TE or AE buffer.
>> The preferred DNA concentration range is 100-250 ng/µl.
>> The A260/A280 ratio should be 1.8-2.0.
>> DNA sample should run on an agarose gel as a single band, showing no degradation, alongside a quantitative DNA marker.
Requirements for DNA quantity – read more.
Requirements for blood samples
>> 2-4 ml of blood with anticoagulant EDTA is needed for genetic analysis. Do not use heparin as an anticoagulant. Make sure the anticoagulant and blood are mixed – do not shake!
>> Blood samples can be preserved at 2-8°C, but for no longer than one week. Do not freeze the blood! Send blood samples by courier service.
Requirements for DNA samples for prenatal testing
>> The sample material is DNA separated from fetal chorionic cells or amniocytes.
>> The amount of DNA required for the test is 4,5 µg.
>> Recommended DNA concentration is 100-250 ng/µl.
>> Analysis results are available in 2-3 weeks.
>> The sample material for excluding maternal DNA contamination, is 2-4ml whole blood with EDTA anticoagulant or 30-50 ng genomic DNA.
Requirements for DNA samples for testing the Microsatellite instability (MSI):
>> The sample materials are tumor- and normal tissue from colon.
>> The best material of tumor tissue is pre-treatment biopsy.
>> The best material of normal tissue is post-treatment resection or 2-4 ml of blood (with anticoagulant EDTA).
>> Storage and transport temperature for paraffin-embedded, formalin-fixed tissue blocks is 20-25°C.
|
