Male Factor Infertility targeted mutation analysis

Genes: CFTR, DDX25, DNAH5, DNAH11, DNAI1, ESR2, FSHB, GNRHR, INSL3, NLRP14, PRDM9, PRM1, PRM2, PRM3, RBMXL2, RXFP2, TEKT2, USP26, UTP14C
47XXY
AZF deletions

No of
detectable
markers:
291 (DNA microarray)
8 (PCR-based test for Y-chromosome deletions)

Lab method: DNA microarray (APEX – Arrayed Primer EXtension), PCR

Price / TAT: Complex MFI test: 492 EUR / 2-4 weeks
Klinefelter syndrome from blood: 87 EUR / 2-4 weeks
Klinefelter syndrome from buccal cells: 87 EUR / 2-4 weeks
Y-chromosome deletions: 87 EUR / 2-4 weeks

Specimen requirements: 2-4 ml of blood with anticoagulant EDTA
Send blood samples at room temperature. Blood samples can be preserved at 2-8°C before shipping. Blood samples are recommended not to freeze and not to store longer than one week.

4 µg DNA in TE, AE or pure sterile water at 100-250 ng/µl
Send DNA samples at room temperature or frozen. To avoid sample loss and contamination, please use 0,5-2,0 ml screw cap tubes, tubes with safe lock lid or wrap the caps of each microtube with parafilm. The A260/A280 ratio should be 1.8-2.0. DNA sample should be run on an agarose gel as a single band, showing no degradation, alongside with a quantitative DNA marker.

Buccal cell sample for assessment of mosaicism in patients with Klinefelter syndrome


Ordering information: Go to online ordering or download sample submission form
Enclose the filled form in the package
Send samples to the following address:

Asper Biotech
Vaksali 17a
Tartu 50410
Estonia